Extension: ancient (Ancient)
A collection of terms appropriate when collecting samples and sequencing samples of data containing ancient nucleic acids, i.e., degraded molecules not from living organisms.
Terms
| MIXS ID | Name | Cardinality and Range | Description |
|---|---|---|---|
| MIXS:999999901 | orig_site_name | * recommended String |
Designated name of the archaeological or ecological site, ancient settlement,... |
| MIXS:999999902 | orig_site_loc | 0..1 String |
The original geographical origin of the sample, when sampled outside its orig... |
| MIXS:999999903 | orig_site_lat | 0..1 String |
The latitude coordinate of the original geographical origin of the sample, e |
| MIXS:999999904 | orig_site_lon | 0..1 String |
The longitude coordinate of the original geographical origin of the sample, e |
| MIXS:999999905 | past_env_broad | 0..1 recommended String |
Report information about the general ancient broad environmental system that ... |
| MIXS:999999906 | past_env_local | 0..1 recommended String |
Report information about the smaller-scale environmental system of the local ... |
| MIXS:999999907 | stratigraph_context | * String |
Associated stratigraphic context(s) that the sample was retrieved from, usual... |
| MIXS:999999908 | column_sample_depth | 0..1 String |
Distance relative to the top or beginning of the sediment core or stratigraph... |
| MIXS:999999909 | context_retrieval_date | 0..1 recommended Datetime |
Date of excavation or retrieval from burial or depositional context, if known |
| MIXS:999999910 | permit_id | * recommended String |
A permit ID, code, or any form of identify provided by any authority (ethical... |
| MIXS:999999911 | permit_authority | * recommended String |
Name of the authorit(ies) or institution(s) that granted sampling and analysi... |
| MIXS:999999912 | permit_date | * recommended Datetime |
Date on which a permit was granted |
| MIXS:999999913 | permit_scope | * recommended String |
Description of the original scope and permissions of the research on the gene... |
| MIXS:999999914 | biocultural_label | * recommended BioCulturalLabelEnum |
Relevant biocultural labels defined by the local contexts project (https://lo... |
| MIXS:999999915 | samp_alt_lab_ids | * String |
An alternative sample or material IDs related to the sample not already cover... |
| MIXS:999999916 | prev_pubs | * String |
Any publications that report data from the same body/skeleton/individual |
| MIXS:999999917 | host_preserv_state | 0..1 String |
Description of the state of the sampled (ancient) organism/host as originally... |
| MIXS:999999918 | storage_conditions | 0..1 String |
General surrounding environmental conditions where the material was stored in... |
| MIXS:999999919 | samp_preserv_treatm | * String |
Description of any treatment applied directly to samples for the specific pur... |
| MIXS:999999920 | cultural_era | 0..1 String |
The cultural era approximating to the period in which the archaeological rema... |
| MIXS:999999921 | geological_epoch | 0..1 GeolEpochEnum |
The geological epoch approximating to the period within which the specimen or... |
| MIXS:999999922 | earliest_chrono_age | 1 recommended Integer |
The maximum/earliest/oldest possible age of a specimen as determined by a dat... |
| MIXS:999999923 | earliest_chrono_sys | 1 recommended ChronoAgeSysEnum |
The reference system associated with the earliest_chrono_age |
| MIXS:999999924 | latest_chrono_age | 1 recommended Integer |
The minimum/latest/youngest possible age of a specimen as determined by a dat... |
| MIXS:999999925 | latest_chrono_sys | 1 recommended ChronoAgeSysEnum |
The reference system associated with the latest_chrono_age |
| MIXS:999999926 | chrono_age_protocol | * recommended ChronoAgeProtocolEnum |
A description of or reference to the methods used to determine the earliest_c... |
| MIXS:999999927 | chrono_age_remarks | 0..1 recommended String |
Notes or comments about the earliest_chrono_age and latest_chrono_age |
| MIXS:999999928 | palaeopath_status | 0..1 String |
Describe briefly any relevant palaeopathological or health-related observatio... |
| MIXS:999999929 | batch_ids | * String |
Identifiers for any form of batch or 'group' that the samples is associated w... |
| MIXS:999999930 | neg_cont_status | 1 recommended Boolean |
Specify whether the sample is a negative control or not |
| MIXS:999999931 | samp_decont_pretreat | * String |
Protocols employed for sample surface decontamination of external modern nuc... |
| MIXS:999999932 | damage_treatment | 1 recommended DamageTreatmentEnum |
Indication of whether characteristic ancient DNA damage has been altered or r... |
| MIXS:999999933 | nucl_acid_extr_date | 0..1 Datetime |
The date when the nucleic acid extraction was started from the sample materia... |
| MIXS:999999934 | experimental_sop | * String |
Provide a DOI or URL to refer to the paper where the field report, nucleic ac... |
| MIXS:999999935 | mid_config | * recommended String |
Index/barcode/primer configuration used during library building for sequencin... |
| MIXS:999999936 | library_name | * recommended String |
Any ID or name used for referring to a nucleic acid sequencing library associ... |
| MIXS:999999937 | lib_polymerase | 0..1 recommended String |
The polymerase enzyme used for building nucleic acid libraries |
| MIXS:999999938 | lib_strandedness | 1..* recommended LibStrandEnum |
The strandedness of the original template nucleic acid molecules used for con... |
| MIXS:999999939 | lib_type | 0..1 recommended LibTypeEnum |
The type of library created, i |
| MIXS:999999940 | lib_preparation_sop | * String |
Citation(s) for the nucleic acid library preparation protocol |
| MIXS:999999941 | num_reamp_cycles | 0..1 Integer |
Number of amplification cycles after library indexing PCR |
| MIXS:999999942 | num_capture_cycles | * Integer |
Number of amplification cycles after capture enrichment |
| MIXS:999999943 | capture_probe_taxid | * Integer |
NCBI taxon ID(s) of all organisms included in the baits of a whole organelle ... |
| MIXS:999999944 | capture_probe_desc | * String |
Description of target enrichment probe designs used (e |
| MIXS:999999945 | desc_read_state | 0..1 recommended String |
Description of the state of the reads in the sequencing data file |
| MIXS:999999946 | data_filt_applied | 0..1 recommended Boolean |
Specify whether associated data was filtered prior to upload, such as host re... |
Annotations
- use_cases: ancient dna samples
LinkML Source
Direct
name: Ancient
annotations:
use_cases:
tag: use_cases
value: ancient dna samples
description: A collection of terms appropriate when collecting samples and sequencing
samples of data containing ancient nucleic acids, i.e., degraded molecules not
from living organisms.
title: ancient
comments:
- This extension that is intended to be used in very specific cases but almost always
in conjunction with other MIxS checklists and extensions. It does not therefore
(currently) include additional common terms from checklists.
from_schema: https://w3id.org/mixs
is_a: Extension
slots:
- orig_site_name
- orig_site_loc
- orig_site_lat
- orig_site_lon
- past_env_broad
- past_env_local
- stratigraph_context
- column_sample_depth
- context_retrieval_date
- permit_id
- permit_authority
- permit_date
- permit_scope
- biocultural_label
- samp_alt_lab_ids
- prev_pubs
- host_preserv_state
- storage_conditions
- samp_preserv_treatm
- cultural_era
- geological_epoch
- earliest_chrono_age
- earliest_chrono_sys
- latest_chrono_age
- latest_chrono_sys
- chrono_age_protocol
- chrono_age_remarks
- palaeopath_status
- batch_ids
- neg_cont_status
- samp_decont_pretreat
- damage_treatment
- nucl_acid_extr_date
- experimental_sop
- mid_config
- library_name
- lib_polymerase
- lib_strandedness
- lib_type
- lib_preparation_sop
- num_reamp_cycles
- num_capture_cycles
- capture_probe_taxid
- capture_probe_desc
- desc_read_state
- data_filt_applied
slot_usage:
orig_site_name:
name: orig_site_name
rank: 1
slot_group: Environment
orig_site_loc:
name: orig_site_loc
rank: 2
slot_group: Environment
orig_site_lat:
name: orig_site_lat
rank: 3
slot_group: Environment
orig_site_lon:
name: orig_site_lon
rank: 4
slot_group: Environment
past_env_broad:
name: past_env_broad
rank: 5
slot_group: Environment
past_env_local:
name: past_env_local
rank: 6
slot_group: Environment
context_retrieval_date:
name: context_retrieval_date
rank: 7
slot_group: Environment
stratigraph_context:
name: stratigraph_context
rank: 8
slot_group: Environment
column_sample_depth:
name: column_sample_depth
rank: 9
slot_group: Environment
permit_id:
name: permit_id
rank: 10
slot_group: Sample body
permit_authority:
name: permit_authority
rank: 11
slot_group: Sample body
permit_date:
name: permit_date
rank: 12
slot_group: Sample body
permit_scope:
name: permit_scope
rank: 13
slot_group: Sample body
biocultural_label:
name: biocultural_label
rank: 14
slot_group: Sample body
samp_alt_lab_ids:
name: samp_alt_lab_ids
rank: 15
slot_group: Sample body
prev_pubs:
name: prev_pubs
rank: 16
slot_group: Sample body
host_preserv_state:
name: host_preserv_state
rank: 17
slot_group: Sample body
storage_conditions:
name: storage_conditions
rank: 18
slot_group: Sample body
samp_preserv_treatm:
name: samp_preserv_treatm
rank: 19
slot_group: Sample body
cultural_era:
name: cultural_era
rank: 20
slot_group: Sample body
geological_epoch:
name: geological_epoch
rank: 21
slot_group: Sample body
earliest_chrono_age:
name: earliest_chrono_age
rank: 22
slot_group: Sample body
earliest_chrono_sys:
name: earliest_chrono_sys
rank: 23
slot_group: Sample body
latest_chrono_age:
name: latest_chrono_age
rank: 24
slot_group: Sample body
latest_chrono_sys:
name: latest_chrono_sys
rank: 25
slot_group: Sample body
chrono_age_protocol:
name: chrono_age_protocol
rank: 26
slot_group: Sample body
chrono_age_remarks:
name: chrono_age_remarks
rank: 27
slot_group: Sample body
palaeopath_status:
name: palaeopath_status
rank: 28
slot_group: Sample body
batch_ids:
name: batch_ids
rank: 29
slot_group: Nucleic acid source
neg_cont_status:
name: neg_cont_status
rank: 30
slot_group: Nucleic acid source
samp_decont_pretreat:
name: samp_decont_pretreat
rank: 31
slot_group: Nucleic acid source
damage_treatment:
name: damage_treatment
rank: 32
slot_group: Nucleic acid source
nucl_acid_extr_date:
name: nucl_acid_extr_date
rank: 33
slot_group: Nucleic acid source
experimental_sop:
name: experimental_sop
rank: 34
slot_group: Nucleic acid source
mid_config:
name: mid_config
rank: 35
slot_group: Sequencing
library_name:
name: library_name
rank: 36
slot_group: Sequencing
lib_polymerase:
name: lib_polymerase
rank: 37
slot_group: Sequencing
lib_strandedness:
name: lib_strandedness
rank: 38
slot_group: Sequencing
lib_preparation_sop:
name: lib_preparation_sop
rank: 39
slot_group: Sequencing
lib_type:
name: lib_type
rank: 40
slot_group: Sequencing
num_reamp_cycles:
name: num_reamp_cycles
rank: 41
slot_group: Sequencing
num_capture_cycles:
name: num_capture_cycles
rank: 42
slot_group: Sequencing
capture_probe_taxid:
name: capture_probe_taxid
rank: 43
slot_group: Sequencing
capture_probe_desc:
name: capture_probe_desc
rank: 44
slot_group: Sequencing
desc_read_state:
name: desc_read_state
rank: 45
slot_group: Data analysis
data_filt_applied:
name: data_filt_applied
rank: 46
slot_group: Data analysis
class_uri: MIXS:9999903
Induced
name: Ancient
annotations:
use_cases:
tag: use_cases
value: ancient dna samples
description: A collection of terms appropriate when collecting samples and sequencing
samples of data containing ancient nucleic acids, i.e., degraded molecules not
from living organisms.
title: ancient
comments:
- This extension that is intended to be used in very specific cases but almost always
in conjunction with other MIxS checklists and extensions. It does not therefore
(currently) include additional common terms from checklists.
from_schema: https://w3id.org/mixs
is_a: Extension
slot_usage:
orig_site_name:
name: orig_site_name
rank: 1
slot_group: Environment
orig_site_loc:
name: orig_site_loc
rank: 2
slot_group: Environment
orig_site_lat:
name: orig_site_lat
rank: 3
slot_group: Environment
orig_site_lon:
name: orig_site_lon
rank: 4
slot_group: Environment
past_env_broad:
name: past_env_broad
rank: 5
slot_group: Environment
past_env_local:
name: past_env_local
rank: 6
slot_group: Environment
context_retrieval_date:
name: context_retrieval_date
rank: 7
slot_group: Environment
stratigraph_context:
name: stratigraph_context
rank: 8
slot_group: Environment
column_sample_depth:
name: column_sample_depth
rank: 9
slot_group: Environment
permit_id:
name: permit_id
rank: 10
slot_group: Sample body
permit_authority:
name: permit_authority
rank: 11
slot_group: Sample body
permit_date:
name: permit_date
rank: 12
slot_group: Sample body
permit_scope:
name: permit_scope
rank: 13
slot_group: Sample body
biocultural_label:
name: biocultural_label
rank: 14
slot_group: Sample body
samp_alt_lab_ids:
name: samp_alt_lab_ids
rank: 15
slot_group: Sample body
prev_pubs:
name: prev_pubs
rank: 16
slot_group: Sample body
host_preserv_state:
name: host_preserv_state
rank: 17
slot_group: Sample body
storage_conditions:
name: storage_conditions
rank: 18
slot_group: Sample body
samp_preserv_treatm:
name: samp_preserv_treatm
rank: 19
slot_group: Sample body
cultural_era:
name: cultural_era
rank: 20
slot_group: Sample body
geological_epoch:
name: geological_epoch
rank: 21
slot_group: Sample body
earliest_chrono_age:
name: earliest_chrono_age
rank: 22
slot_group: Sample body
earliest_chrono_sys:
name: earliest_chrono_sys
rank: 23
slot_group: Sample body
latest_chrono_age:
name: latest_chrono_age
rank: 24
slot_group: Sample body
latest_chrono_sys:
name: latest_chrono_sys
rank: 25
slot_group: Sample body
chrono_age_protocol:
name: chrono_age_protocol
rank: 26
slot_group: Sample body
chrono_age_remarks:
name: chrono_age_remarks
rank: 27
slot_group: Sample body
palaeopath_status:
name: palaeopath_status
rank: 28
slot_group: Sample body
batch_ids:
name: batch_ids
rank: 29
slot_group: Nucleic acid source
neg_cont_status:
name: neg_cont_status
rank: 30
slot_group: Nucleic acid source
samp_decont_pretreat:
name: samp_decont_pretreat
rank: 31
slot_group: Nucleic acid source
damage_treatment:
name: damage_treatment
rank: 32
slot_group: Nucleic acid source
nucl_acid_extr_date:
name: nucl_acid_extr_date
rank: 33
slot_group: Nucleic acid source
experimental_sop:
name: experimental_sop
rank: 34
slot_group: Nucleic acid source
mid_config:
name: mid_config
rank: 35
slot_group: Sequencing
library_name:
name: library_name
rank: 36
slot_group: Sequencing
lib_polymerase:
name: lib_polymerase
rank: 37
slot_group: Sequencing
lib_strandedness:
name: lib_strandedness
rank: 38
slot_group: Sequencing
lib_preparation_sop:
name: lib_preparation_sop
rank: 39
slot_group: Sequencing
lib_type:
name: lib_type
rank: 40
slot_group: Sequencing
num_reamp_cycles:
name: num_reamp_cycles
rank: 41
slot_group: Sequencing
num_capture_cycles:
name: num_capture_cycles
rank: 42
slot_group: Sequencing
capture_probe_taxid:
name: capture_probe_taxid
rank: 43
slot_group: Sequencing
capture_probe_desc:
name: capture_probe_desc
rank: 44
slot_group: Sequencing
desc_read_state:
name: desc_read_state
rank: 45
slot_group: Data analysis
data_filt_applied:
name: data_filt_applied
rank: 46
slot_group: Data analysis
attributes:
orig_site_name:
name: orig_site_name
annotations:
Expected_value:
tag: Expected_value
value: Name of site or location where sample was originated
description: Designated name of the archaeological or ecological site, ancient
settlement, or location etc. where the sample was originally collected. Can
be a non-geographical name, such as a field-specific name or code, the official
name of an excavation, or a colloquial name that is used in academic literature.
Typically names that would not be found on official maps. If the site name is
unclear please use the name of the closest location or region as best as possible.
Can also include different transliterations or languages used in the literature.
title: Name of site or location where sample originated
examples:
- value: Valley of the Kings
- value: Krakow Spadzista B
- value: Coopers Cave
- value: Cutler Fossil Site
- value: Kap København Formation
- value: Northern Italy, Lombardy, exact location unknown
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 1
keywords:
- environment
- sample
slot_uri: MIXS:999999901
alias: orig_site_name
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: true
multivalued: true
orig_site_loc:
name: orig_site_loc
annotations:
Expected_value:
tag: Expected_value
value: Name of original geographic origin of the sample
description: The original geographical origin of the sample, when sampled outside
its original natural environment (e.g. sampled in a museum collection), as defined
by the country or sea name followed by specific region name. Country or sea
names should be chosen from the INSDC country list (http://insdc.org/country.html),
or the GAZ ontology (http://purl.bioontology.org/ontology/GAZ).
title: original site location
examples:
- value: 'South Africa: Western Cape'
- value: 'Germany: Baden-Württemberg, Geißenklösterle Cave'
- value: 'Northern Italy: Lombardy'
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 2
keywords:
- location
- site
slot_uri: MIXS:999999902
alias: orig_site_loc
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: false
multivalued: false
structured_pattern:
syntax: '^{country}: {region}, {specific_location}$'
orig_site_lat:
name: orig_site_lat
description: The latitude coordinate of the original geographical origin of the
sample, e.g. the original place the sample was buried, deposited, or formed.
In cases where the sample was directly sampled in the burial environment for
the purposes of scientific investigation, this will be the same as geo_loc_name,
and lat_lon. For samples kept in collections, the geo_loc_name and lat_lon
terms are used to refer to the collection where the sample is stored, but this
term is used for the original geographic location the sample existed in prior
to archiving in a collection (i.e., should correspond to orig_site_loc, not
the collection itself as recorded in site_name). The values should be reported
in decimal degrees, limited to 8 decimal points, and in WGS84 system.
title: original geographic location (latitude)
examples:
- value: '50.586825'
- value: '-0.123'
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 3
slot_uri: MIXS:999999903
alias: orig_site_lat
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: false
multivalued: false
structured_pattern:
syntax: ^{lat}$
interpolated: true
partial_match: true
orig_site_lon:
name: orig_site_lon
description: The longitude coordinate of the original geographical origin of the
sample, e.g. the original place the sample was buried, deposited, or formed.
In cases where the sample was directly sampled in the burial environment for
the purposes of scientific investigation, this will be the same as geo_loc_name,
and lat_lon. For samples kept in collections, the geo_loc_name and lat_lon
terms are used to refer to the collection where the sample is stored, but this
term is used for the original geographic location the sample existed in prior
to archiving in a collection (i.e., should correspond to orig_site_loc, not
the collection itself, as recorded in site_name). The values should be reported
in decimal degrees, limited to 8 decimal points, and in WGS84 system.
title: original geographic location (longitude)
examples:
- value: '6.408977'
- value: '-12.12'
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 4
slot_uri: MIXS:999999904
alias: orig_site_lon
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: false
multivalued: false
structured_pattern:
syntax: ^{lon}$
interpolated: true
partial_match: true
past_env_broad:
name: past_env_broad
description: 'Report information about the general ancient broad environmental
system that the sample or specimen existed or lived within, as it was at the
time of deposition or burial (e.g. in the desert or a forest). This should not
describe the environment as it is today (e.g. farmland), but specifically the
state as it was in the past (i.e. the palaeo- or (pre)historical ecosystem).
Compared to `env_broad_scale` which is taken from direct observation, the information
about the past environment will normally be derived from inference from archaeological,
palaeontological, geological, or other scientific methods. We recommend using
subclasses of EnvO s biome class: http://purl.obolibrary.org/obo/ENVO_00000428.
EnvO documentation about how to use the field for present day equivalents: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS"'
title: broad-scale past environmental context
examples:
- value: dessert biome [ENVO:01000247]
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 5
keywords:
- context
- environmental
- ancient
slot_uri: MIXS:999999905
alias: past_env_broad
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: true
pattern: ^([^\s-]{1,2}|[^\s-]+.+[^\s-]+) \[[a-zA-Z]{2,}:[a-zA-Z0-9]\d+\]$
structured_pattern:
syntax: ^{termLabel} \[{termID}\]$
interpolated: true
partial_match: true
past_env_local:
name: past_env_local
annotations:
Expected_value:
tag: Expected_value
value: Report information about smaller environmental entities having causal
influences upon the sample or specimen at/during the time of burial
description: 'Report information about the smaller-scale environmental system
of the local vicinity of the sample or specimen at the time of deposition or
burial (e.g. in hillside, burial mound, or midden). This should not describe
the environment as it is today (e.g. carpark), but specifically the entity or
entities surrounding the sample that may have significant causal influences
as it was in the past. Compared to `env_local_scale` which is taken from direct
observation, the information about the past environment will normally be derived
from inference from archaeological, palaeontological, geological, or other scientific
methods. We recommend using EnvO terms which are of smaller than your entry
for past_env_broad. Terms, such as anatomical sites, from other OBO Library
ontologies which interoperate with EnvO (e.g. UBERON) are accepted in this field.
EnvO documentation about how to use the field for present day equivalents: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS"'
title: local past environmental context
examples:
- value: hillside [ENVO:01000333]
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 6
keywords:
- context
- environmental
- ancient
string_serialization: '{termLabel} [{termID}]'
slot_uri: MIXS:999999906
alias: past_env_local
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: true
stratigraph_context:
name: stratigraph_context
annotations:
Expected_value:
tag: Expected_value
value: Stratigraphic context that the sample was retrieved from
description: Associated stratigraphic context(s) that the sample was retrieved
from, usually from an archaeological or palaeontological excavation. Description(s)
or identifier(s) of stratigraphic units or layer names within and/or relative
to the site (e.g., stratigraphic unit ID, archaeological feature ID, layer name
or a description, grid location).
title: stratigraphic context
examples:
- value: Layer 5
- value: Layer UE
- value: Horizon IIc, Quadrant 77
- value: HF_23447_77_410_IIc
- value: KrSp C2/2011/B5
- value: US10
- value: YSL
- value: Subunit 1
- value: Black Mousterian (BM)
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 8
keywords:
- identifiers
- excavation
slot_uri: MIXS:999999907
alias: stratigraph_context
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: false
multivalued: true
column_sample_depth:
name: column_sample_depth
annotations:
Expected_value:
tag: Expected_value
value: depth of the sample in the column of a stratigraphy or sediment core
description: 'Distance relative to the top or beginning of the sediment core or
stratigraphic sequence (defined by the term ''depth'') from which the sample
was taken. This is not necessarily the same as the depth from floor level or
Mean Sea Level (MSL) (which should be defined using the term: depth), as a core
could start part way below the surface. Can also be a range if the depth of
the sample is not precisely measured.'
title: sample depth in column
examples:
- value: 34 cm
- value: 23 - 56 cm
- value: 100 in
in_subset:
- environment
from_schema: https://w3id.org/mixs
rank: 9
keywords:
- environment
- core
- sediment
slot_uri: MIXS:999999908
alias: column_sample_depth
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: string
required: false
recommended: false
multivalued: false
structured_pattern:
syntax: ^{scientific_float}( *- *{scientific_float})? *{text}$
interpolated: true
partial_match: true
context_retrieval_date:
name: context_retrieval_date
annotations:
Preferred_unit:
tag: Preferred_unit
value: year
description: 'Date of excavation or retrieval from burial or depositional context,
if known. If excavations were done during a longer period, report its midpoint
at a month or year level. In case no exact time is available, the date can be right
truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10;
2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant.'
title: date of retrieval from depositional context
examples:
- value: '2023'
- value: 1972-11
- value: '2001-09-25'
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 7
slot_uri: MIXS:999999909
alias: context_retrieval_date
owner: Ancient
domain_of:
- Ancient
slot_group: Environment
range: datetime
required: false
recommended: true
permit_id:
name: permit_id
description: A permit ID, code, or any form of identify provided by any authority
(ethical, local, legal, academic etc.) associated with the approval of the analysis
of this particular sample, if available.
title: permit or approval ID
examples:
- value: DE-123-JK
in_subset:
- investigation
from_schema: https://w3id.org/mixs
rank: 10
keywords:
- ethics
slot_uri: MIXS:999999910
alias: permit_id
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: true
multivalued: true
permit_authority:
name: permit_authority
annotations:
Expected_value:
tag: Expected_value
value: Name of authority providing permission or ethical approval.
description: Name of the authorit(ies) or institution(s) that granted sampling
and analysis (e.g. human remains) and/or export permission (e.g. animal remains),
as well any form of ethical approval (whether from institutional research ethics
boards such as REB or IRBs, or indigenous or native community associations),
if available.
title: permit authority
examples:
- value: University of Copenhagen
- value: Federal Foreign Office (Germany)
in_subset:
- investigation
from_schema: https://w3id.org/mixs
rank: 11
keywords:
- ethics
- location
slot_uri: MIXS:999999911
alias: permit_authority
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: true
multivalued: true
permit_date:
name: permit_date
description: 'Date on which a permit was granted. The date can be right truncated
i.e. all of these are valid times: 2008-01-23; 2008-01; 2008; Except: 2008-01;
2008 all are ISO8601 compliant.'
title: date of permit approval
examples:
- value: '2023-12-01'
in_subset:
- investigation
from_schema: https://w3id.org/mixs
rank: 12
slot_uri: MIXS:999999912
alias: permit_date
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: datetime
required: false
recommended: true
multivalued: true
permit_scope:
name: permit_scope
annotations:
Expected_value:
tag: Expected_value
value: Description of the scope of ethical permissions for data use.
description: Description of the original scope and permissions of the research
on the genetic material, as was approved by a legal, ethical, or relevant authority
(e.g. bacteria only, DNA only, bacteria and human, no host read analysis allowed).
Note this description is only informative, and will not necessarily automatically
apply restrictions to associated data to other researchers.
title: permit scope
examples:
- value: Defined scope only includes the study of bacterial sequences and any
human sequence is not covered under the agreement.
in_subset:
- ethics
from_schema: https://w3id.org/mixs
rank: 13
keywords:
- ethics
string_serialization: '{text}'
slot_uri: MIXS:999999913
alias: permit_scope
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: true
multivalued: true
biocultural_label:
name: biocultural_label
annotations:
Expected_value:
tag: Expected_value
value: Relevant biocultural label from https://localcontexts.org/labels/biocultural-labels/
description: Relevant biocultural labels defined by the local contexts project
(https://localcontexts.org/label/bc-provenance/) that describe in what ways
this data can be reused, as permitted by any associated native or indigenous
peoples or communities.
title: biocultural label
examples:
- value: BC R
- value: BC MC
- value: BC MC;BC CV
in_subset:
- ethics
from_schema: https://w3id.org/mixs
rank: 14
keywords:
- ethics
slot_uri: MIXS:999999914
alias: biocultural_label
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: BioCulturalLabelEnum
required: false
recommended: true
multivalued: true
samp_alt_lab_ids:
name: samp_alt_lab_ids
description: An alternative sample or material IDs related to the sample not already
covered by terms samp_name and source_mat_id, including from associated other
non-genetic analyses of the same sample (e.g. museum IDs, internal lab sample
ID from sampling such a bone drilling). Can be specified multiple times for
different ID contexts.
title: alternative sample IDs
examples:
- value: ABC_24
- value: Grave 6
- value: 'Museum ID: NHM_AR_123, Drilling ID: DRL_001, Extraction ID: ABC_24'
in_subset:
- investigation
from_schema: https://w3id.org/mixs
rank: 15
string_serialization: '{text}'
slot_uri: MIXS:999999915
alias: samp_alt_lab_ids
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
multivalued: true
prev_pubs:
name: prev_pubs
description: Any publications that report data from the same body/skeleton/individual
title: previous publications
examples:
- value: doi:10.1016/j.jas.2015.02.0181
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 16
slot_uri: MIXS:999999916
alias: prev_pubs
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
multivalued: true
pattern: ^^PMID:\d+$|^doi:10.\d{2,9}/.*$|^https?:\/\/(?:www\.)?[-a-zA-Z0-9@:%._\+~#=]{1,256}\.[a-zA-Z0-9()]{1,6}\b(?:[-a-zA-Z0-9()@:%_\+.~#?&\/=]*)$$
structured_pattern:
syntax: ^{PMID}|{DOI}|{URL}$
interpolated: true
partial_match: true
host_preserv_state:
name: host_preserv_state
annotations:
Expected_value:
tag: Expected_value
value: Description of the preservation of the sampled (ancient) organism/host
at time/immediately after death.
description: Description of the state of the sampled (ancient) organism/host as
originally preserved in the burial environment at the time of or immediately
following death. This can be both natural or artificial, such as mummification
or burning for funerary purposes.
title: preservation state of sampled organism/host at/immediately after death
examples:
- value: complete artificial mummification.
- value: natural partial mummification.
- value: fully skeletonised.
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 17
keywords:
- ancient
string_serialization: '{text}'
slot_uri: MIXS:999999917
alias: host_preserv_state
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
multivalued: false
storage_conditions:
name: storage_conditions
description: General surrounding environmental conditions where the material was
stored in long-term collection storage prior sampling for nucleic acid analysis that
may influence nucleic acid recovery or library construction. For example, specify
temperature, humidity, presence of microbial overgrowth etc..
title: storage conditions
examples:
- value: climate-controlled
- value: Mould growth in storage box observed
- value: Stored at -20oC until 2025-05-15
- value: Stored in the museum from 1924 to 2021
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 18
string_serialization: '{text}'
slot_uri: MIXS:999999918
alias: storage_conditions
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
samp_preserv_treatm:
name: samp_preserv_treatm
description: Description of any treatment applied directly to samples for the
specific purpose of maximising longevity of sample preservation in archives
and/or collections by curators that may influence downstream nucleic acid recovery
or library construction, such as storage fluid or reconstructive glue.
title: preservational treatment
examples:
- value: stored in formalin
- value: reconstructive glue applied
- value: alcohol preserved
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 19
keywords:
- ancient
string_serialization: '{text}'
slot_uri: MIXS:999999919
alias: samp_preserv_treatm
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
multivalued: true
cultural_era:
name: cultural_era
annotations:
Expected_value:
tag: Expected_value
value: chronotology or PeriodO term; text
description: The cultural era approximating to the period in which the archaeological
remains existed in. Where possible use terms from ontologies such as Chronontology
(https://chronontology.dainst.org/) or PeriodO (https://perio.do/en/).
title: cultural era or period
examples:
- value: 'Copper Age [Chronotology: NW6hofAScJSE]'
- value: Upper Middle Palaeolthic
- value: Not collected
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 20
keywords:
- ancient
- age
string_serialization: '{termLabel} [{termID}]|{text}'
slot_uri: MIXS:999999920
alias: cultural_era
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
multivalued: false
geological_epoch:
name: geological_epoch
annotations:
Expected_value:
tag: Expected_value
value: ontology term; text
description: 'The geological epoch approximating to the period within which the
specimen or sample existed. Where possible use terms from ontologies. NOTE:
This term is for geological timescales. For more precise or anthropogenic defined
periods, use `Cultural Era`.'
title: geological epoch
examples:
- value: Pleistocene
- value: Upper Cretaceous
- value: Pliocene
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 21
keywords:
- ancient
- age
slot_uri: MIXS:999999921
alias: geological_epoch
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: GeolEpochEnum
required: false
recommended: false
multivalued: false
earliest_chrono_age:
name: earliest_chrono_age
annotations:
Expected_value:
tag: Expected_value
value: age value corresponding to unit
description: The maximum/earliest/oldest possible age of a specimen as determined
by a dating method. If multiple dating measurements available, use the most
earliest/oldest date to provide widest range of age possibilities. The specific
age unit should be specified by the term earliest_chrono_sys. More information
on specific dates (e.g. radiocarbon lab codes) can be specified in the term
chrono_age_remarks.
title: Earliest Chronometric Age
examples:
- value: '120000'
- value: '1900'
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
broad_mappings:
- chrono:earliestChronometricAge
rank: 22
slot_uri: MIXS:999999922
alias: earliest_chrono_age
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: integer
required: true
recommended: true
multivalued: false
earliest_chrono_sys:
name: earliest_chrono_sys
description: The reference system associated with the earliest_chrono_age.
title: Earliest Chronometric Age Reference System
examples:
- value: cal BP
- value: CE
- value: Ma
- value: ka
in_subset:
- investigation
from_schema: https://w3id.org/mixs
broad_mappings:
- chrono:earliestChronometricAgeReferenceSystem
rank: 23
slot_uri: MIXS:999999923
alias: earliest_chrono_sys
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: ChronoAgeSysEnum
required: true
recommended: true
multivalued: false
latest_chrono_age:
name: latest_chrono_age
annotations:
Expected_value:
tag: Expected_value
value: age value corresponding to unit
description: The minimum/latest/youngest possible age of a specimen as determined
by a dating method. If multiple dating measurements available, use the most
latest/youngest date to provide widest range of age possibilities. The specific
age unit should be specified by the term latest_chrono_sys. More information
on specific dates (e.g. radiocarbon lab codes) can be specified in the term
chrono_age_remarks.
title: Latest Chronometric Age
examples:
- value: '100000'
- value: '1700'
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
broad_mappings:
- chrono:latestChronometricAge
rank: 24
slot_uri: MIXS:999999924
alias: latest_chrono_age
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: integer
required: true
recommended: true
multivalued: false
latest_chrono_sys:
name: latest_chrono_sys
description: The reference system associated with the latest_chrono_age.
title: Latest Chronometric Age Reference System
examples:
- value: cal BP
- value: CE
- value: Ma
- value: ka
in_subset:
- investigation
from_schema: https://w3id.org/mixs
broad_mappings:
- chrono:latestChronometricAgeReferenceSystem
rank: 25
slot_uri: MIXS:999999925
alias: latest_chrono_sys
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: ChronoAgeSysEnum
required: true
recommended: true
multivalued: false
chrono_age_protocol:
name: chrono_age_protocol
description: A description of or reference to the methods used to determine the
earliest_chrono_age and latest_chrono_age.
title: Chronometric Age Protocol
examples:
- value: radiocarbon dating
- value: optically stimulated infrared luminescence
- value: contextual dating
- value: historical records
in_subset:
- investigation
from_schema: https://w3id.org/mixs
broad_mappings:
- chrono:chronometricAgeProtocol
rank: 26
slot_uri: MIXS:999999926
alias: chrono_age_protocol
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: ChronoAgeProtocolEnum
required: false
recommended: true
multivalued: true
chrono_age_remarks:
name: chrono_age_remarks
description: Notes or comments about the earliest_chrono_age and latest_chrono_age.
For more detail use Chronometric Age Protocol to point to original publication
describing method. Useful to specify confidence and/or accuracy of reported
date.
title: Chronometric Age Remarks
examples:
- value: radiocarbon dating, calibrated with OxCal v4.3 with 95% confidence interval
- value: based on proxy dating from other bone samples of stratigraphic layer
- value: a coin found in the burial was from the 3rd century was found in the
mouth of the skeleton
- value: age taken from previous publication Doe et al. 2019
- value: 'radiocarbon age ID: OxA-12345'
in_subset:
- investigation
from_schema: https://w3id.org/mixs
close_mappings:
- chrono:chronometricAgeRemarks
rank: 27
slot_uri: MIXS:999999927
alias: chrono_age_remarks
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: true
multivalued: false
palaeopath_status:
name: palaeopath_status
annotations:
Expected_value:
tag: Expected_value
value: description of health related observation on ancient remains
description: Describe briefly any relevant palaeopathological or health-related
observations of the remains of the individual under study.
title: palaeopathology status
examples:
- value: Osteoporosis.
- value: Parasites found in pelvic area.
- value: Caries on right upper molar.
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 28
keywords:
- palaeopathology
- host health
- ancient
string_serialization: '{text}'
slot_uri: MIXS:999999928
alias: palaeopath_status
owner: Ancient
domain_of:
- Ancient
slot_group: Sample body
range: string
required: false
recommended: false
multivalued: false
batch_ids:
name: batch_ids
annotations:
Expected_value:
tag: Expected_value
value: list any batch_ids the sample, nucleic acids, or library was associated
with during processing and sequencing
description: 'Identifiers for any form of batch or ''group'' that the samples
is associated with, examples including: individual/skeleton ID, sediment core
IDs, extract batch, library batch, sequencing run etc.. These IDs should always
act as ''umbrella'' IDs that allow association with entries processed together,
to allow for downstream analyses of batch effects. Ideally, the information
should indicate or specify what type of batch the ID is describing.'
title: batch identifiers
examples:
- value: EXTB1;LIBB2;SeqRun1
- value: 'Extraction batch: 1'
- value: 20260501-A2 (extraction);20202607-B2 (library)
- value: ExtrMG;LibMG;SeqMG
- value: 'Core: North-East Greenland 73G'
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 29
keywords:
- identifiers
slot_uri: MIXS:999999929
alias: batch_ids
owner: Ancient
domain_of:
- Ancient
slot_group: Nucleic acid source
range: string
required: false
recommended: false
multivalued: true
neg_cont_status:
name: neg_cont_status
description: Specify whether the sample is a negative control or not.
title: negative control status
examples:
- value: 'true'
- value: 'false'
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 30
keywords:
- control
slot_uri: MIXS:999999930
alias: neg_cont_status
owner: Ancient
domain_of:
- Ancient
slot_group: Nucleic acid source
range: boolean
required: true
recommended: true
samp_decont_pretreat:
name: samp_decont_pretreat
description: Protocols employed for sample surface decontamination of external modern
nucleic acids; Treatment used on the samples immediately prior to nucleic acid
extraction. Dependant on the sample type. More relevant for bones than environmental
samples. E.g. buffers, EDTA, etc.
title: sample decontamination pretreatment
examples:
- value: doi:10.1016/j.jas.2015.02.0181
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 31
slot_uri: MIXS:999999931
alias: samp_decont_pretreat
owner: Ancient
domain_of:
- Ancient
slot_group: Nucleic acid source
range: string
multivalued: true
pattern: ^^PMID:\d+$|^doi:10.\d{2,9}/.*$|^https?:\/\/(?:www\.)?[-a-zA-Z0-9@:%._\+~#=]{1,256}\.[a-zA-Z0-9()]{1,6}\b(?:[-a-zA-Z0-9()@:%_\+.~#?&\/=]*)$$
structured_pattern:
syntax: ^{PMID}|{DOI}|{URL}$
interpolated: true
partial_match: true
damage_treatment:
name: damage_treatment
annotations:
Expected_value:
tag: Expected_value
value: enumeration
description: Indication of whether characteristic ancient DNA damage has been
altered or removed from a DNA extract in a laboratory. If damage has been removed,
but whether it was fully or partially removed is unknown (e.g. with UDG treatment)
- specify 'other', and describe known information in `experimental_sop` and
related free text descriptive terms.
title: Damage treatment type
examples:
- value: none
- value: partial-removal
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 32
keywords:
- ancient
slot_uri: MIXS:999999932
alias: damage_treatment
owner: Ancient
domain_of:
- Ancient
slot_group: Nucleic acid source
range: DamageTreatmentEnum
required: true
recommended: true
multivalued: false
nucl_acid_extr_date:
name: nucl_acid_extr_date
description: 'The date when the nucleic acid extraction was started from the sample
material. In case no exact time is available, the date can be right truncated
i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10;
2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant'
title: date of extraction of nucleic acids from sample
examples:
- value: '2023-12-01'
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 33
slot_uri: MIXS:999999933
alias: nucl_acid_extr_date
owner: Ancient
domain_of:
- Ancient
slot_group: Nucleic acid source
range: datetime
required: false
recommended: false
experimental_sop:
name: experimental_sop
description: Provide a DOI or URL to refer to the paper where the field report,
nucleic acid extraction, library construction, and other procedures are explained
in more detail, e.g. the paper reporting the data.
title: experimental procedures
examples:
- value: doi:10.1093/nar/gkr771
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 34
slot_uri: MIXS:999999934
alias: experimental_sop
owner: Ancient
domain_of:
- Ancient
slot_group: Nucleic acid source
range: string
required: false
recommended: false
multivalued: true
pattern: ^^PMID:\d+$|^doi:10.\d{2,9}/.*$|^https?:\/\/(?:www\.)?[-a-zA-Z0-9@:%._\+~#=]{1,256}\.[a-zA-Z0-9()]{1,6}\b(?:[-a-zA-Z0-9()@:%_\+.~#?&\/=]*)$$
structured_pattern:
syntax: ^{PMID}|{DOI}|{URL}$
interpolated: true
partial_match: true
mid_config:
name: mid_config
annotations:
Expected_value:
tag: Expected_value
value: Description of the indexing configuration of the library
description: Index/barcode/primer configuration used during library building for
sequencing. This includes information such as the number, type and location
of indexes, the index/primer kit/list, or if 'inline' barcodes or UMIs were
ligated directly onto the template molecules.
title: multiplex identifiers or indexing configuration of libraries
examples:
- value: dual index with single internal barcode.
- value: UDI index sequences.
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 35
keywords:
- library
- preparation
string_serialization: '{text}'
slot_uri: MIXS:999999935
alias: mid_config
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: string
required: false
recommended: true
multivalued: true
library_name:
name: library_name
annotations:
Expected_value:
tag: Expected_value
value: name of sequencing library
description: Any ID or name used for referring to a nucleic acid sequencing library
associated with the sample.
title: library name
examples:
- value: JK1234
- value: JFC001.A0101
- value: A003-1-SG1
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 36
keywords:
- sequencing
slot_uri: MIXS:999999936
alias: library_name
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: string
required: false
recommended: true
multivalued: true
lib_polymerase:
name: lib_polymerase
annotations:
Expected_value:
tag: Expected_value
value: name of polymerase used during library construction
description: The polymerase enzyme used for building nucleic acid libraries.
title: library polymerase
examples:
- value: Agilent PfuTurbo Cx HotStart
- value: KAPA HiFi HotStart
- value: AmpliTaq Gold DNA Polymerase
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 37
keywords:
- polymerase
- library
- preparation
string_serialization: '{text}'
slot_uri: MIXS:999999937
alias: lib_polymerase
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: string
required: false
recommended: true
lib_strandedness:
name: lib_strandedness
annotations:
Expected_value:
tag: Expected_value
value: nucleic acid library strandedness
description: The strandedness of the original template nucleic acid molecules
used for constructing the sequencing library
title: nucleic acid strandedness in library creation
examples:
- value: single
- value: double
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 38
keywords:
- library
- preparation
slot_uri: MIXS:999999938
alias: lib_strandedness
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: LibStrandEnum
required: true
recommended: true
multivalued: true
lib_type:
name: lib_type
description: The type of library created, i.e., amplicon, enriched, or shotgun.
An amplicon library is a library that has been amplified to target a single
specific region of a genome (e.g. a specific gene). An enriched library is
a library has had a particular genome, or multiple genomic regions/positions
'captured' or enriched typically via baits/probes. A shotgun library has undergone
no type of targeted amplification/enrichment for a particular genomic region
or genome, i.e., random sequencing of any nucleic acid molecule contained in
a genomic library.
title: library type
examples:
- value: shotgun
- value: amplicon
- value: enriched
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 40
keywords:
- library
- preparation
slot_uri: MIXS:999999939
alias: lib_type
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: LibTypeEnum
required: false
recommended: true
lib_preparation_sop:
name: lib_preparation_sop
description: Citation(s) for the nucleic acid library preparation protocol.
title: library preparation protocols
examples:
- value: doi:10.1093/nar/gkr771
in_subset:
- nucleic acid sequence source
from_schema: https://w3id.org/mixs
rank: 39
slot_uri: MIXS:999999940
alias: lib_preparation_sop
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: string
required: false
recommended: false
multivalued: true
pattern: ^^PMID:\d+$|^doi:10.\d{2,9}/.*$|^https?:\/\/(?:www\.)?[-a-zA-Z0-9@:%._\+~#=]{1,256}\.[a-zA-Z0-9()]{1,6}\b(?:[-a-zA-Z0-9()@:%_\+.~#?&\/=]*)$$
structured_pattern:
syntax: ^{PMID}|{DOI}|{URL}$
interpolated: true
partial_match: true
num_reamp_cycles:
name: num_reamp_cycles
description: Number of amplification cycles after library indexing PCR. If capture
data, this refers to amplifications prior to the capture experiments.
title: number of reamplification cycles
examples:
- value: '12'
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 41
slot_uri: MIXS:999999941
alias: num_reamp_cycles
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: integer
num_capture_cycles:
name: num_capture_cycles
description: Number of amplification cycles after capture enrichment
title: number of reamplification cycles after capture
examples:
- value: '12'
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 42
slot_uri: MIXS:999999942
alias: num_capture_cycles
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: integer
required: false
recommended: false
multivalued: true
capture_probe_taxid:
name: capture_probe_taxid
annotations:
Expected_value:
tag: Expected_value
value: Taxonomic IDs corresponding to organism(s) included in target enrichment
(a.k.a. capture) probe design
description: NCBI taxon ID(s) of all organisms included in the baits of a whole
organelle or whole genome-level capture panel. There should be an (ideally)
species level taxonomic ID entry for each organism that had sequences included
in the design. If whole genera were targeted, you can instead use a single genus
level taxonomic ID or that of any relevant higher taxonomic unit.
title: genomic capture probe taxonomic IDs
examples:
- value: '9606'
- value: '632'
- value: '9789'
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 43
keywords:
- sequencing
- library
- enrichment
- capture
slot_uri: MIXS:999999943
alias: capture_probe_taxid
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: integer
required: false
recommended: false
multivalued: true
capture_probe_desc:
name: capture_probe_desc
annotations:
Expected_value:
tag: Expected_value
value: Description of probe set used for target enrichment/library selection
(a.k.a. capture)
description: Description of target enrichment probe designs used (e.g., species
included, sequences, type, company). This can include custom kits (please provide
a general description and DOI if available) or commercially available kit (provide
ID and company).
title: enrichment probe design description
examples:
- value: Custom probe design (70 bp biotinylated RNA probes) covering 500 full
E. Coli genomes; company TE-Science
- value: Commercial RNA baits kit ABC001-EC from company TE-Science, purchased
2020
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 44
keywords:
- library
- enrichment
string_serialization: '{text}'
slot_uri: MIXS:999999944
alias: capture_probe_desc
owner: Ancient
domain_of:
- Ancient
slot_group: Sequencing
range: string
required: false
recommended: false
multivalued: true
desc_read_state:
name: desc_read_state
annotations:
Expected_value:
tag: Expected_value
value: description of any modifications to data away from original raw files
description: Description of the state of the reads in the sequencing data file.
Describe any in silico processing or modification of the sequencing reads away
from the original state as received from the sequencer. This should include
details such as adapter-, barcode-, and/or quality- trimming, or any filtering
such as for read length or of off-target reads.
title: description of read state
examples:
- value: Adapter trimmed, quality filtered, and host reads removed through mapping
for ethical reasons.
- value: Adapters removed by Trimmomatic (v0.39), and off-target reads removed
after mapping to the HG19 Human reference with bwa aln (v0.7.19).
- value: Demultiplexed with bcl2fastq, inline barcodes removed, and reads quality
filtered with fastp (v1.0.0).
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 45
keywords:
- data analysis
- data
slot_uri: MIXS:999999945
alias: desc_read_state
owner: Ancient
domain_of:
- Ancient
slot_group: Data analysis
range: string
required: false
recommended: true
multivalued: false
data_filt_applied:
name: data_filt_applied
annotations:
Expected_value:
tag: Expected_value
value: Whether associated data was filtered
description: Specify whether associated data was filtered prior to upload, such
as host reads removal.
title: Data filtering applied
examples:
- value: 'no'
- value: 'yes'
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
rank: 46
keywords:
- data analysis
slot_uri: MIXS:999999946
alias: data_filt_applied
owner: Ancient
domain_of:
- Ancient
slot_group: Data analysis
range: boolean
required: false
recommended: true
multivalued: false
class_uri: MIXS:9999903